You may qualify for this study if you meet the following criteria.

Inclusion Criteria:

• Age 18-45
• BMI between 18-30
• English proficiency
• Access to a mobile phone
• Regular menstrual cycle (if female)
• Willing to use non-hormonal birth control during study participation (if female)
• In the last 30 days, used cannabis at least 5 days/week on average
• Current major depressive episode

Exclusion Criteria:

• Certain psychiatric disorders
• Some medications (determined by the researcher) that may interfere with study outcomes
• Recent drug use other than cannabis
• Anemia or other blood or clotting-related disorders
• High blood pressure
• Currently seeking treatment for substance use
• Pregnant, nursing, or planning to become pregnant
• Suicidal behavior or suicidal ideation with intent in the past 6 months
• Severe depression

The study involves 1 screening visit and 3 in-person laboratory visits over the course of one week. You will complete phone surveys multiple times daily asking about your mood and withdrawal symptoms. Blood is drawn multiple times at the laboratory visits to assess endocannabinoid levels. The third laboratory day includes a stress test in order to examine stress response.

Participants are asked to not use cannabis for 3 days, starting the evening before the second visit. Participants will complete saliva drug tests twice daily during this period to ensure abstinence.

Laboratory visits begin at 8:30am, last 4-6 hours, and are in-person at the MUSC campus in downtown Charleston. Visit 1 will take place on a Friday, Visit 2 the following Monday, and then Visit 3 on Wednesday of that week.

Compensation of up to $720 is provided for participation.

There is no cost for you to participate in our research study.